2E83
T31V mutant of FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.615, 84.394, 40.387 |
| Unit cell angles | 90.00, 93.33, 90.00 |
Refinement procedure
| Resolution | 26.330 - 1.520 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.097 | 0.122 |
| Number of reflections | 37400 | |
| <I/σ(I)> | 25.26 | 8.29 |
| Completeness [%] | 99.1 | 92.9 |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 6000, sodium acetate, Tris, pH 7.5, VAPOR DIFFUSION, temperature 298K |
