2E7Q

Crystal structure of basic winged bean lectin in complex with b blood group trisaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.548
Spacegroup name	P 21 21 2
Unit cell lengths	157.410, 90.545, 73.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.740 - 2.750
R-factor	0.198
Rwork	0.198
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB RNTRY 1WBL
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.131	0.500
Number of reflections	27614
<I/σ(I)>	12.7	2.1
Completeness [%]	96.9	92.5
Redundancy	3.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.05	293	6-7%(w/v) PEG 6000, 10%(v/v) isopropenol, 20mM PBS, pH 7.05, VAPOR DIFFUSION, HANGING DROP, temperature 293K