2E7D

Crystal structure of a NEAT domain from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	124.247, 124.247, 119.655
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.250 - 2.200
R-factor	0.24
Rwork	0.240
R-free	0.26500
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	18212
Completeness [%]	99.9	100
Redundancy	11.1	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.25	293	0.1M sodium acetate, 2.2M ammonium sulfate, pH 4.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.25	293	0.1M sodium acetate, 2.2M ammonium sulfate, pH 4.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K