2E6X
X-ray structure of TT1592 from Thermus thermophilus HB8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-11 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9, 0.97910, 0.97941 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.486, 56.051, 48.885 |
Unit cell angles | 90.00, 93.48, 90.00 |
Refinement procedure
Resolution | 35.360 - 2.000 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.25300 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.360 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.061 | 0.192 |
Number of reflections | 17159 | |
<I/σ(I)> | 13.7 | 3.86 |
Completeness [%] | 97.4 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.6M potassium thiocyanate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |