2E6L
structure of mouse WRN exonuclease domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 93.15 |
| Detector technology | CCD |
| Collection date | 2005-02-14 |
| Wavelength(s) | 1.2835 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.047, 59.147, 60.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.570 - 2.200 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 11712 | |
| <I/σ(I)> | 40 | 40 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
