2E6K
X-ray structure of Thermus thermopilus HB8 TT0505
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9, 0.97905, 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.610, 88.960, 117.180 |
| Unit cell angles | 72.48, 89.13, 73.36 |
Refinement procedure
| Resolution | 38.590 - 2.090 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.590 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.061 | 0.190 |
| Number of reflections | 154606 | |
| <I/σ(I)> | 9.9 | 4.7 |
| Completeness [%] | 97.7 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 298 | 10%(w/v) PEG 4000, 0.1M HEPES, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
