2E6E
Crystal structure of the stationary phase survival protein SurE from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-07 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.222, 68.635, 220.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.630 - 2.500 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e6c |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.065 | 0.133 |
Number of reflections | 31508 | |
<I/σ(I)> | 26.9 | 13.1 |
Completeness [%] | 96.0 | 80.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.2 | 293 | 30% ethylene glycol, 1% PEG4000, 0.1M Na acetate, pH 4.2, VAPOR DIFFUSION, temperature 293K |