2E64
Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii, Mutations R48A and K111A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.334, 82.713, 72.737 |
| Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
| Resolution | 36.770 - 1.500 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.20300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB code 2E3Y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.770 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.041 | 0.251 |
| Number of reflections | 68646 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 97.5 | 93.9 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.2 | 295 | PEG20K, Acetate, NaOH, ATP, Biotin, pH 5.2, microbatch, temperature 295K |
