2E5Y

Epsilon subunit and ATP complex of F1F0-ATP synthase from the Thermophilic Bacillus PS3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-18
Detector	Bruker DIP-6040
Spacegroup name	P 21 21 21
Unit cell lengths	37.480, 64.917, 103.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.920
R-factor	0.20211
Rwork	0.200
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aqt
RMSD bond length	0.016
RMSD bond angle	2.208
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.920
Number of reflections	17451
Completeness [%]	92.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	5% PEG 6000, 0.1M citric acid, pH 4.0, Vapor diffusion, hanging drop, temperature 293.0K