2E5V

Crystal structure of L-Aspartate Oxidase from hyperthermophilic archaeon Sulfolobus tokodaii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-02
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.970, 103.256, 163.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.090
Rwork	0.194
R-free	0.20900
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	87.370
High resolution limit [Å]	2.090
Number of reflections	56198

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.6	293	23% PEG 8000, 0.1M Tris/HCl, 0.2M MgCl2, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K