2E5L
A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-10-01 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 0.98010 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 411.790, 411.790, 173.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 148.830 - 3.300 |
| Rwork | 0.259 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 148.830 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.162 | 0.755 |
| Number of reflections | 214963 | |
| <I/σ(I)> | 7.48 | 2.5 |
| Completeness [%] | 97.1 | 95.2 |
| Redundancy | 6.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 10mM HEPES-KOH, 10mM MgCl2, 60mM NH4Cl, 6mM beta-mercaptoethanol, 11-12% MPD, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES-KOH | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | NH4Cl | ||
| 4 | 1 | 1 | beta-mercaptoethanol | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | HEPES-KOH | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | NH4Cl | ||
| 9 | 1 | 2 | MPD |
