2E50

Crystal structure of SET/TAF-1beta/INHAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	60.913, 64.435, 124.421
Unit cell angles	90.00, 90.85, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.22
Rwork	0.218
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.499
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.250	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.048	0.420
Number of reflections	54757
<I/σ(I)>	12.3
Completeness [%]	96.8	93.4
Redundancy	3.1	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	2.75M Ammonium sulfate, 0.1M sodium citrate buffer, 0.2M potassium/sodium tartrate, 30mM MgCl2, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	2.75M Ammonium sulfate, 0.1M sodium citrate buffer, 0.2M potassium/sodium tartrate, 30mM MgCl2, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	2.75M Ammonium sulfate, 0.1M sodium citrate buffer, 0.2M potassium/sodium tartrate, 30mM MgCl2, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K