2E4Y
Crystal structure of the extracellular region of the group II metabotropic glutamate receptor complexed with 2R,4R-APDC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.393, 91.799, 112.972 |
Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
Resolution | 12.000 - 3.400 |
R-factor | 0.2282 |
Rwork | 0.224 |
R-free | 0.28360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e4u |
RMSD bond length | 0.009 |
RMSD bond angle | 1.441 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.089 | 0.500 |
Number of reflections | 23883 | |
<I/σ(I)> | 5.1 | 1.4 |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.2M NH4H2PO4, 2.5-5.0% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |