2E4T
Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000, 1.2824, 0.9800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.077, 59.155, 170.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.960 |
| R-factor | 0.13332 |
| Rwork | 0.133 |
| R-free | 0.14606 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.788 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.990 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Number of reflections | 292562 | |
| <I/σ(I)> | 22.1 | 2.32 |
| Completeness [%] | 98.9 | 91.7 |
| Redundancy | 5.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 14% PEG 3350, 0.1M MES-NaOH pH 5.8, 6mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
