2E4N
Mutant V251M structure of PH0725 from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-09-06 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.199, 104.199, 138.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 1.800 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wng |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.044 | 0.537 |
Number of reflections | 71276 | |
<I/σ(I)> | 15.9 | 4.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.2 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbach | 5.5 | 295 | 3.85M Na Formate, 0.1M Acetate, 1mM DTT, pH 5.5, Microbach, temperature 295K |