2E4L
Thermodynamic and Structural Analysis of Thermolabile RNase HI from Shewanella oneidensis MR-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Collection date | 2006-03-09 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 77.128, 77.128, 76.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.220 - 2.000 |
| R-factor | 0.19017 |
| Rwork | 0.189 |
| R-free | 0.21851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rn2 |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.314 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.096 | 0.518 |
| Number of reflections | 43245 | |
| <I/σ(I)> | 23.5 | 2.61 |
| Completeness [%] | 99.7 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | VAPOR DIFFUSION, SITTING DROP, temperature 277K |
