2E4G
RebH with bound L-Trp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 62 |
| Unit cell lengths | 114.750, 114.750, 231.233 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.800 - 2.080 |
| Rwork | 0.186 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aqj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.269 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.800 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 102800 | |
| <I/σ(I)> | 7.8 | 2.6 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 277 | 1.2M Na/K phosphate, pH 7.0, hanging drop, temperature 277K |
