2E4F
Crystal Structure of the Cytoplasmic Domain of G-Protein-Gated Inward Rectifier Potassium Channel Kir3.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2005-12-06 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 77.173, 77.173, 87.307 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.302 |
Rwork | 0.244 |
R-free | 0.27890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n9p |
RMSD bond length | 0.008 |
RMSD bond angle | 1.450 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.274 | 2.380 |
High resolution limit [Å] | 2.302 | 2.302 |
Rmerge | 0.021 | 0.466 |
Number of reflections | 12201 | |
<I/σ(I)> | 15.5 | 4.93 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 9.4 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1M HEPES-NaOH, 0.2M MgCl2, 30%(v/v) iso-propanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |