2E48
Crystal Structure of Human D-Amino Acid Oxidase: Substrate-Free Holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-24 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 149.652, 181.727, 50.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.21284 |
| Rwork | 0.210 |
| R-free | 0.25929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2du8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.347 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.102 | 0.245 |
| Number of reflections | 31102 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 97.3 | 82.9 |
| Redundancy | 3.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG 4000, 0.1M sodium citrate, 0.2M ammonium acetate, 10% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
