2E46

Crystal Structure Analysis of the clock protein EA4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-08
Detector	MAR CCD 165 mm
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	59.500, 59.500, 112.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.760 - 2.300
R-factor	0.21229
Rwork	0.208
R-free	0.28522
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e9p
RMSD bond length	0.036
RMSD bond angle	2.902
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Number of reflections	9519
Completeness [%]	99.9
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	9% ethlene glycol, 1M ammonium fluoride, 100mM Bis-Tris, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K