2E43
Crystal structure of C/EBPbeta Bzip homodimer K269A mutant bound to A High Affinity DNA fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-29 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.681, 112.731, 74.932 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.070 - 2.100 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gu4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.075 | |
Number of reflections | 25384 | |
Completeness [%] | 99.3 | 94.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | magnesium chloride, Tris-Hcl, PEG4000, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | magnesium chloride | ||
2 | 1 | 1 | Tris-Hcl | ||
3 | 1 | 1 | PEG4000 | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | magnesium chloride | ||
6 | 1 | 2 | Tris-Hcl | ||
7 | 1 | 2 | PEG4000 | ||
8 | 1 | 2 | H2O |