2E42
Crystal structure of C/EBPbeta Bzip homodimer V285A mutant bound to A High Affinity DNA fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-02 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.881, 113.319, 74.439 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.420 - 1.800 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gu4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | |
Number of reflections | 39915 | |
Completeness [%] | 98.8 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | magnesium chloride, MES, PEG400, PH 6, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | magnesium chloride | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | PEG400 | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | magnesium chloride | ||
6 | 1 | 2 | MES | ||
7 | 1 | 2 | PEG400 | ||
8 | 1 | 2 | H2O |