2E3S
Crystal structure of CERT START domain co-crystallized with C24-ceramide (P21)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.243, 75.646, 42.327 |
Unit cell angles | 90.00, 108.44, 90.00 |
Refinement procedure
Resolution | 40.160 - 1.940 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.115 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.940 | 1.950 |
Number of reflections | 17894 | |
Completeness [%] | 98.1 | 83.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP |