2E3D
Crystal structure of E. coli glucose-1-phosphate uridylyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-12 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.900, 109.300, 100.500 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.202 |
| Rwork | 0.198 |
| R-free | 0.24300 |
| Structure solution method | SIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.500 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | SOLVE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 106815 | |
| <I/σ(I)> | 13.7 | 3.2 |
| Completeness [%] | 98.5 | 96.5 |
| Redundancy | 6.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2.0 M ammomium sulfate, 0.2M lithium chloride, 0.1M MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
