2E3B
Crystal structure of the HA-bound form of Arthromyces ramosus peroxidase at 1.3 Angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.71 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 73.150, 73.150, 114.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 1.300 |
R-factor | 0.1484 |
Rwork | 0.141 |
R-free | 0.19870 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.012 |
RMSD bond angle | 0.031 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | SHELX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 45.620 | 1.370 |
High resolution limit [Å] | 1.300 | 4.110 | 1.300 |
Rmerge | 0.068 | 0.047 | 0.279 |
Total number of observations | 37068 | 69481 | |
Number of reflections | 78980 | ||
<I/σ(I)> | 2.6 | 11.3 | 0.8 |
Completeness [%] | 99.4 | 99.7 | 97.4 |
Redundancy | 8 | 13.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.5 | 297 | 37% saturated ammonium sulfate, Tris-HCl, pH 7.5, MICRODIALYSIS, temperature 297K |