2E3A
Crystal structure of the NO-bound form of Arthromyces ramosus peroxidase at 1.3 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.71 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 73.770, 73.770, 115.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.300 |
| R-factor | 0.1528 |
| Rwork | 0.148 |
| R-free | 0.20050 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.028 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.066 | 0.264 |
| Total number of observations | 67847 | |
| Number of reflections | 78980 | |
| <I/σ(I)> | 3.2 | 0.8 |
| Completeness [%] | 98.3 | 92.9 |
| Redundancy | 7.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 297 | 37% saturated ammonium sulfate, Tris-HCl, pH 7.5, MICRODIALYSIS, temperature 297K |
