2E2X

Sec14 Homology Module of Neurofibromin in complex with phosphatitylethanolamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9537
Spacegroup name	P 41 21 2
Unit cell lengths	110.100, 110.100, 121.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.26
Rwork	0.254
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d4q
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.070
Number of reflections	52887
<I/σ(I)>		4.1
Completeness [%]	97.9	95.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	5% PEG 4000, 0.25M NaPP, MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 291K