2E2V
Substrate Schiff-base analogue of copper amine oxidase from Arthrobacter globiformis formed with benzylhydrazine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-01 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 158.054, 62.640, 183.951 |
| Unit cell angles | 90.00, 112.16, 90.00 |
Refinement procedure
| Resolution | 26.523 - 1.800 |
| Rwork | 0.217 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.217 |
| RMSD bond angle | 1.380 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.523 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.071 | 0.071 |
| Number of reflections | 175433 | |
| Completeness [%] | 99.2 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | 0.3mM benzylhydrazine for 12h and at 310K, 10mg/ml enzyme solution, 1.05M potassium tartrate, 25mM HEPES at 289 K., pH 6.8, MICRODIALYSIS |
