2E2U
Substrate Schiff-base analogue of copper amine oxidase from Arthrobacter globiformis formed with 4-hydroxybenzylhydrazine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-07 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 157.967, 63.070, 184.052 |
Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
Resolution | 26.565 - 1.680 |
Rwork | 0.209 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.354 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.565 | 1.770 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.058 | 0.058 |
Number of reflections | 189031 | |
Completeness [%] | 98.9 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.8 | 289 | 0.3mM 4-hydroxybenzylhydrazine for 12h and at 310K, 10mg/ml enzyme solution, 1.05M potassium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |