2E2E
TPR domain of NrfG mediates the complex formation between heme lyase and formate-dependent nitrite reductase in Escherichia Coli O157:H7
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Detector technology | CCD |
| Collection date | 2005-05-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950, 0.97956, 0.97171 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.802, 85.824, 49.073 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.050 |
| R-factor | 0.23499 |
| Rwork | 0.231 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 22119 | |
| Completeness [%] | 98.7 | 95.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | ||||
| 1 | ||||
| 1 |
