2E2C

E2-C, AN UBIQUITIN CONJUGATING ENZYME REQUIRED FOR THE DESTRUCTION OF MITOTIC CYCLINS

Replaces:  1E2CReplaces:  1BR7

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1996-10
Detector	RIGAKU
Spacegroup name	P 31 2 1
Unit cell lengths	46.334, 46.334, 150.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.216
Rwork	0.216
R-free	0.29700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1AAK
RMSD bond length	0.007
RMSD bond angle	23.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.000	2.000
Rmerge	0.061	0.358 *
Number of reflections	13327
<I/σ(I)>	11.7	3.7
Completeness [%]	99.4	92.7
Redundancy	4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *		pH 7.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30 (%)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	lithium sulfate	0.2 (M)