2E2A
ASP81LEU ENZYME IIA FROM THE LACTOSE SPECIFIC PTS FROM LACTOCOCCUS LACTIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-03-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.960, 87.960, 79.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| Rwork | 0.198 |
| R-free | 0.24900 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1e2a |
| RMSD bond length | 0.176 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 18726 | |
| <I/σ(I)> | 13.7 | 2.7 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | THE PROTEIN WAS CRYSTALLIZED FROM 0.9M NA ACETATE AND 0.1M NA CACODYLATE AT PH 6.5. HANGING-DROP METHOD WAS USED. THE INITIAL CONCENTRATION OF THE PROTEIN IN THE DROP WAS 5 MG/ML. |
