2E24
crystal structure of a mutant (R612A) of xanthan lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.807, 90.230, 78.098 |
| Unit cell angles | 90.00, 97.56, 90.00 |
Refinement procedure
| Resolution | 46.900 - 2.150 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.113 | 0.324 |
| Number of reflections | 39353 | |
| Completeness [%] | 97.3 | 77.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 22% PEG 4000, 0.2M ammonium formate, 0.1M Sodium Bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
