2E22
Crystal structure of xanthan lyase in complex with mannose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2005-12-20 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.358, 91.462, 159.653 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.150 - 2.400 |
R-factor | 0.165 |
Rwork | 0.165 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j0m |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.450 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.064 | 0.254 |
Number of reflections | 29738 | |
Completeness [%] | 88.6 | 42.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 22% PEG 4000, 0.2M ammonium formate, 0.1M sodium Bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |