2E1V
Crystal structure of Dendranthema morifolium DmAT, seleno-methionine derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-03-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97909, 0.97928, 0.95000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.777, 122.908, 70.332 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 30.460 - 1.800 |
| R-factor | 0.19753 |
| Rwork | 0.196 |
| R-free | 0.23376 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.104 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 | 0.276 |
| Number of reflections | 81316 | |
| <I/σ(I)> | 31.4 | 5.3 |
| Completeness [%] | 98.6 | 93 |
| Redundancy | 7.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 20% glycerol, 0.2M magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
