2E1E

Crystal structure of the HRDC Domain of Human Werner Syndrome Protein, WRN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2006-07-01
Detector	RIGAKU
Wavelength(s)	1.00
Spacegroup name	I 41 2 2
Unit cell lengths	64.458, 64.458, 189.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.253
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1D8B and 1WUD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	9021
Completeness [%]	96.8	77.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	293	pH 9.5, VAPOR DIFFUSION, temperature 293K