2E1C
Structure of Putative HTH-type transcriptional regulator PH1519/DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 68.410, 92.350, 109.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.000 - 2.100 |
| Rwork | 0.206 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ri7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.127 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.456 |
| Number of reflections | 20389 | |
| <I/σ(I)> | 5.6 | 1.6 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 875mM ammonium sulfate, 10mM magnesium chloride, 20mM sodium chloride, 100mM MES buffer, 5% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 10 | 1 | 2 | MES | ||
| 11 | 1 | 2 | glycerol | ||
| 12 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | magnesium chloride | ||
| 3 | 1 | 1 | sodium chloride | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | glycerol | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | ammonium sulfate | ||
| 8 | 1 | 2 | magnesium chloride | ||
| 9 | 1 | 2 | sodium chloride |
