2E1A
crystal structure of FFRP-DM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97925 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.800, 95.800, 95.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 81.000 - 2.500 |
Rwork | 0.190 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z4p |
RMSD bond length | 0.007 |
RMSD bond angle | 1.379 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.092 | 0.299 |
Number of reflections | 35886 | |
<I/σ(I)> | 7.7 | 2.5 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 278 | 1.0M LiCl, 10% PEG 6000, 100mM citrate, 20% glycerol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |