2E11
The Crystal Structure of XC1258 from Xanthomonas campestris: A CN-hydrolase Superfamily Protein with an Arsenic Adduct in the Active Site
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97905, 0.96108 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 143.280, 154.305, 51.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.830 - 1.730 |
| R-factor | 0.18636 |
| Rwork | 0.140 |
| R-free | 0.21746 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.345 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.830 | 104.830 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.140 | 0.140 |
| Number of reflections | 112277 | |
| <I/σ(I)> | 9 | 9.1 |
| Completeness [%] | 98.4 | 95.3 |
| Redundancy | 5.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
