2E0Z

Crystal structure of virus-like particle from Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-11
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	P 41 21 2
Unit cell lengths	631.500, 631.500, 351.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.600
Rwork	0.268
R-free	0.26700
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.132	0.844
Number of reflections	773334
<I/σ(I)>	8.8	1.6
Completeness [%]	96.8	97.5
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		16~20% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	7.5		16~20% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP