2E0W
T391A precursor mutant protein of gamma-Glutamyltranspeptidase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 134.600, 134.600, 118.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.550 |
R-factor | 0.22 |
Rwork | 0.217 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | A MOLECULE (CHAIN ID A B) OF PDB ENTRY 2DBU |
RMSD bond length | 0.010 |
RMSD bond angle | 1.282 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.092 | 0.314 |
Number of reflections | 33506 | |
<I/σ(I)> | 11.2 | |
Completeness [%] | 92.7 | 91.9 |
Redundancy | 12 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 18% PEG 4000, 10% iso-propanol, 0.1M sodium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |