2E0S
Carbohydrate recognition of C-terminal half of lactoferrin: Crystal structure of the complex of C-lobe with rhamnose at 2.15 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.511, 50.352, 65.896 |
| Unit cell angles | 90.00, 107.88, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.18091 |
| Rwork | 0.180 |
| R-free | 0.20338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nkx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.674 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 21564 | |
| <I/σ(I)> | 6.4 | 2.1 |
| Completeness [%] | 98.8 | 90.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M MES, 25% POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 0.01M ZINC SULPHATE, pH 6.5, VAPOR DIFFUSION, temperature 298K |
