2E0Q
Crystal structure of K53E thioredoxin from Sulfolobus tokodaii strain7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.951, 52.123, 33.794 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.490 |
| R-factor | 0.17294 |
| Rwork | 0.172 |
| R-free | 0.19185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.583 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.490 |
| Number of reflections | 57836 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 16% PEG-3000, 0.1M acetate (pH 4.1), 25% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
