2DZZ

Crystal structure of N392V mutant of yeast bleomycin hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	95
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 63 2 2
Unit cell lengths	152.480, 152.480, 89.434
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.020 - 2.150
R-factor	0.19
Rwork	0.190
R-free	0.23200
Structure solution method	isomorphous replacement
RMSD bond length	0.015
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.150
Number of reflections	29770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	14-20% PEG 4K, 100mM Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K