2DZU
Structure of mutant tryptophan synthase alpha-subunit (D110N) from a hyperthermophile, Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.254, 77.474, 166.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.460 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1geq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.500 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.023 | 0.148 |
| Number of reflections | 17530 | |
| <I/σ(I)> | 18 | 8 |
| Completeness [%] | 99.2 | 90.4 |
| Redundancy | 5.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 0.1M MES-NAOH, 10-15% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
