2DZO
Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-01-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 |
Unit cell lengths | 99.599, 99.599, 73.082 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NAS6P-RPT3 COMPLEX P21 FORM |
RMSD bond length | 0.020 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.053 | 0.390 |
Number of reflections | 16358 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.8 | 298 | PEG6K, MES, pH 6.80, VAPOR DIFFUSION, temperature 298K |