2DZN
Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-05-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.376, 100.220, 72.203 |
Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | GANKYRIN |
RMSD bond length | 0.012 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.056 | 0.203 |
Number of reflections | 43936 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | PEG6K, MES, pH 6.50, VAPOR DIFFUSION, temperature 298K |