2DZE
Crystal structure of histone chaperone Asf1 in complex with a C-terminus of histone H3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-07-14 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 40.155, 52.130, 60.654 |
Unit cell angles | 107.35, 105.94, 93.95 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.21514 |
Rwork | 0.214 |
R-free | 0.24352 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cu9 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.750 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.056 | 0.191 |
Number of reflections | 38738 | |
Completeness [%] | 94.1 | 91.4 |
Redundancy | 2.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG6000, AS, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |