2DZC
Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii, Mutation R48A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.358, 82.766, 72.712 |
| Unit cell angles | 90.00, 103.23, 90.00 |
Refinement procedure
| Resolution | 33.530 - 1.450 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.23600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2dxu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.048 | 0.379 |
| Number of reflections | 72200 | |
| <I/σ(I)> | 15.5 | 3.2 |
| Completeness [%] | 92.3 | 97.2 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.2 | 295 | PEG20K, Acetate, NaOH, pH 5.2, microbatch, temperature 295K |
