2DYW
A Backbone binding DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 175 |
| Detector technology | CCD |
| Collection date | 2003-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.675, 41.692, 65.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.500 - 1.130 |
| Rwork | 0.182 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 355D(NDB entry: bdl084) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.022 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.070 |
| Number of reflections | 21375 |
| Completeness [%] | 90.1 |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.19mM [d(CGCGAATTCGCG)]2, 0.19mM TriplatinNC, 31mM sodium cacodylate (pH 6.5), 9.6mM magnesium chloride, 6.9% 2-methyl-2,4-pentanediol (MPD), equilibrated against a reservoir of 30% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | TriplatinNC | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | sodium cacodylate | ||
| 7 | 1 | 2 | magnesium chloride | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
